Fig. 3. Feasibility, sensitivity and specificity of AMC-SDA. (a) Real-time fluorescence and (b) PAGE image for three target DNAs (10–11 M) of different lengths. (c) Real-time fluorescence caused by different concentrations of target DNA (TA-1). (d) Schematic showing the positions of mismatching and the sites where the cleavage/extension occurs in forward initiation. (e) Real-time fluorescence caused by matched and single-base mismatched sequences (10–11 M).
