Fig. 4. Universality of AMC-SDA. (a and b) Real-time fluorescence for various concentrations of target sequences from HIV Gag gene and HBV S gene, respectively. (c) Schematic of a target fragment in plasmid pUC57 and the cleavage sites mediated by various forward/reverse aligners. (d) Real-time fluorescence for a 56-bp fragment from no. 1240 to 1296 base of pUC57. (e) PAGE image showing the amplification products corresponding to (d) (Left) and three fragments with different length from plasmid pUC57 when using various forward aligners/primers (Right).