Scheme 1. Schematic of AMC based on nicking endonuclease Nt.BstNBI. (a) Recognition sequence and cleavage site of Nt.BstNBI. (b) Basic structure of the proposed DNA aligner and working principle of AMC. Nt.BstNBI firstly binds to the recognition sequence in the stem of DNA aligner (Loading), and is aligned with a specific locus through the hybridization of the two side arms with target DNA (Localization), then cleaves at that site (Reaction). (c) Programmable, sequence-specific cleavage of DNA via AMC. By simply varying the sequences of the aligner's side arms, a break can be made at any locus of target DNA.
