Scheme 2. Schematic illustration of AMC-SDA. (Step 1) Target DNA, or its antisense sequence, hybridizes with DNA aligner and is cleaved by Nt.BstNBI through AMC. (Step 2) The cleaved sequence with a definite 3′-end binds a linear primer, followed by polymerase-catalyzed extension along this linear primer to generate a complete double-stranded recognition site. (Step 3) Nt.BstNBI binds to the newly formed recognition site and makes a nick four bases downstream. (Step 4) Polymerase catalyzes the extension from the nicking site, displacing the previous strand. Step 3 and Step 4 can be repeated many times.
