Abca4−/−Rdh8−/− mice (1-month-old) were exposed to light at 10,000 lux for 30 min. A. Various tissues including the eye, liver, heart, brain, spleen, lung and kidney were collected 24 h after light exposure. RNA levels are presented as fold change over non-light exposed control tissues. B. Immunoblot of LCN2 protein in light exposed 1-month-old Abca4−/−Rdh8−/− mice 1, 3, and 7 days after light exposure. C. Quantitative analyses of the immunoblot after normalization to β-actin are presented. D. Serum was collected from 1-month-old Abca4−/−Rdh8−/− mice at different time points after light exposure. E. Immunohistochemistry of LCN2 protein in the retinal sections of 1-month-old Abca4−/−Rdh8−/− mice 24 h after light exposure. Scale bar: 50 μm. RPE, retinal pigmented epithelium; ONL, outer nuclear layer; INL, inner nuclear layer; GCL ganglion cell layer. Error bars indicate S.D. of the means, n=3, *p < 0.05.