Table 1.

Rate and equilibrium constants calculated by multivariate data fitting.

Constant	DL-1-SO3	LD-1-SO3	DD-1-SO3
Kgs (mM−1)	1.31×101	2.19×101	4.52×101
k̃cat (mM·min−1)	2.63×10−2	2.78×10−1	6.38×10−3
KM (mM)	20.3	31.1	6.58
ζ	0.96	1.13	1.98
kin (min−1)	5.06×10−2	5.06×10−2	5.06×10−2
kout (min−1)	0.79×10−2	0.79×10−2	0.79×10−2
δM	0.83	0. 83	0. 83
δC	15.7	15.7	15.7
kk0 (min−1)	2.11×10−3	2.97×10−3	6.29×10−3
σ (mM−1)	0.48	1.24	3.69
[GC]tr (mM)	2.99	2.29	1.30

K_gs: equilibrium constant for the gelation process; k̃_cat: catalytic rate constant; K_M: Michaelis-Menten constant; ζ: gel-specific constant representing the average number of empty sites in the gel structure per molecule of hydrogelator available for the inclusion of one precursor; k_in and k_out: transport rate constants; δ^M: ratio of enzyme concentration in the culture medium to enzyme concentration used in the hydrolysis experiment; δ^C: ratio of enzyme concentration in the cell to enzyme concentration used in the hydrolysis experiment. [G^C]_tr (a threshold value), k_k⁰ (a plateau value) and σ (slope) of the killing efficacy curve. Known parameters: k_d: HeLa cell doubling rate constant which is 4.81×10⁻⁴ min⁻¹; k_g: gelation rate constant which is 10⁵ min⁻¹.