Fig. 1. Sequential deuteration of complex 1 monitored by NMR and MS. (a) 1H NMR spectrum of 1 after reaction with AgNO3 in 60% MeOD-d4/40% D2O for 24 h, and FT-ICR mass spectrum after 72 h. (b) FT-ICR mass spectra of 1 treated with 0.95 mol equiv. AgNO3 and incubated at ambient temperature in 60% MeOD-d4/40% D2O. Isotopic pattern at t = 0 min corresponds to 1, and at t = 20, 40, 60 and 80 min to the sum of isotopic patterns for 1 at various stages of deuteration.
