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. 2018 Apr 14;27(5):923–932. doi: 10.1002/pro.3397

Figure 5.

Figure 5

Cbl linker‐RING domains alone require phosphorylation for ubiquitylation activity. Coomassie‐stained gels showing the results of ubiquitylation assays using constructs without the TKB module, that is, the linker‐RING domains alone (see diagram of construct at top). Two lanes are shown for all experiments, for S. rosetta Cbl and C. elegans Cbl, they are t = 0 (left lane) and 15 min (right). For D. melanogaster Cbl TKB‐RING, the lanes are t = 0 (left) and t = 30 min (right). The bands corresponding to the Cbl proteins are marked with asterisks. Similar to Figure 4, a higher molecular weight smear, indicated by an arrow, is only seen in the presence of Src‐KD (M w ∼ 35 kDa; right gel panels) and not the Src‐inactive protein (M w ∼ 52 kDa; left gel panels).