Fig. 3. (A) Confocal fluorescence images of HeLa cells incubated with POP (4 μM, 30 min) in PBS at 37 °C and 4 °C, or pre-treated with 2-DG (150 mM, 45 min) and oligomycin (5 μM, 45 min) in DMEM at 37 °C and then treated with POP (4 μM, 30 min) in PBS at 37 °C after being washed with PBS three times. (B) Average fluorescence intensities from the images shown in (A). For the green channel, the emission was collected at 500–540 nm (λex = 488 nm); for the red channel, the emission was collected at 650–700 nm (λex = 561 nm). Scale bar: 50 μm.
