Fig. 4. (A) Confocal fluorescence images of HeLa cells pre-treated with various endocytotic inhibitors [amiloride (20 μg mL–1, 30 min), chlorpromazine (10 μg mL–1, 30 min) and MβCD (7.5 mM, 30 min)] and OATP inhibitor BSP (250 μM, 5 min) in DMEM and then treated with POP (4 μM) in PBS for 30 min at 37 °C after being washed with PBS three times. (B) Average fluorescence intensities from images shown in (A). For the green channel, the emission was collected at 500–540 nm (λex = 488 nm); for the red channel, the emission was collected at 650–700 nm (λex = 561 nm). Scale bar: 50 μm.
