Skip to main content
. 2018 Feb 22;3(4):e93029. doi: 10.1172/jci.insight.93029

Figure 7. Chronic albuterol exposure reduces forskolin/IBMX–stimulated generation of cAMP in wtCFTR+ and F508del-CFTR+ CFBE41o- cells.

Figure 7

wtCFTR+ and F508del-CFTR+ CFBE41o- cells were pretreated with albuterol (10 μM) for 72 hours, then stimulated to produce cAMP with 10 μM forskolin/100 μM IBMX (10 minutes). cAMP levels were then analyzed by colorimetric ELISA. wtCFTR+ (A, n = 6) and F508del-CFTR+ (B, n = 6) CFBE41o- cells produce minimal cAMP in the absence of either pretreatment or stimulation (open circles). Stimulation with 10 μM forskolin/100 μM IBMX in untreated cells leads to cAMP production (open squares), which is reduced by more than 60% in cells pretreated for 72 hours with albuterol (open triangles). All studies are internally normalized to control conditions to allow for comparisons. Both experiments are representative of studies repeated in duplicate with similar results. Data presented represent the mean ± SEM. n = 6 wells per condition. ****P < 0.0005 by 1-way ANOVA with Tukey’s multiple comparisons test.