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. 2018 Feb 22;3(4):e95824. doi: 10.1172/jci.insight.95824

Figure 13. WISP-1 regulates autophagy signaling in human coronary artery endothelial cells (HCAECs).

Figure 13

Equal numbers of HCAECs were seeded overnight. The following day, cells were treated with 0 ng/ml, 5 ng/ml, 10 ng/ml, or 20 ng/ml recombinant WISP-1 (rec.WISP-1) for 72 hours and then assessed for autophagy markers ATG 5-ATG 12 complex, ATG-7, and LC3BII (17 and 14 kDa) and GAPDH (loading control). Graphs represent densitometry analyses of Western blots. n = 4 per group. Results depicted as mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. P values obtained by 1-way ANOVA with Bonferroni’s post test.