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. 2018 Feb 22;3(4):e95824. doi: 10.1172/jci.insight.95824

Figure 9. WISP-1 enhances proangiogenic and prosurvival genotypes in human coronary artery endothelial cells (HCAECs).

Figure 9

Equal numbers of HCAECs were seeded overnight. The following day, cells were treated with 0 ng/ml, 5 ng/ml, 10 ng/ml, or 20 ng/ml recombinant WISP-1 (rec.WISP-1) for 72 hours and then assessed for VEGF-A, Rho-A, RAC, the ratio of phospho-BAD/total BAD, and GAPDH (loading control) via Western blot. Graphs show quantitated densitometry differences normalized to loading control (GAPDH). Data is representative of 4 repeated analyses; results depicted as mean ± SEM. *P ≤ 0.05 relative to control, **P ≤ 0.01, ***P ≤ 0.001. P values obtained by 1-way ANOVA with Bonferroni’s post test.