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. 2018 Feb 22;3(4):e97228. doi: 10.1172/jci.insight.97228

Figure 1. Interaction of PTEN and serum response factor (SRF) in the nucleus of medial smooth muscle cells (SMCs).

Figure 1

Proximity ligation assay (PLA) and confocal microscopy were used to detect PTEN-SRF interactions in medial SMCs of human nonatherosclerotic coronary arteries. Representative images shown. (A) Left: PTEN positive control using mouse anti-PTEN primary antibody and anti-mouse PLUS and anti-mouse MINUS PLA probe demonstrates nuclear and cytoplasmic expression of PTEN. Right: SRF positive control using rabbit anti-SRF primary antibody and anti-rabbit PLUS and anti-rabbit MINUS PLA probe demonstrates predominantly nuclear expression of SRF. Scale bar: 20 μm. (B) PLA using mouse anti-PTEN and rabbit anti-SRF primary antibodies and anti-mouse PLUS and anti-rabbit MINUS PLA probe demonstrates PTEN-SRF nuclear interactions in medial SMCs of human nonatherosclerotic hyperplasia coronary arteries, but no interactions in adventitial cells (arrowheads; right panel). M = media; A = adventitia; white dashed line in right panel indicates the external elastic lamina. Scale bars: 50 μm. (C) PLA negative controls for SRF (left) and PTEN (right) demonstrate lack of signal when either primary antibodies are omitted. Scale bars: 50 μm. For all panels: Red = positive PLA; Blue = DAPI for cell nuclei.