Figure 5.

Ovaries of virgin queens 5 days after the initiation of the alate separation assay. (a) Ovaries of virgin alates kept in queenright colonies are small and nonvitellogenic. (b) and (c) Virgins were kept for 5 days in queenless colonies. In (b), ovaries of virgins that did not dealate remained small, similar to those shown in (a) for queenright condition. In (c) virgins dealated within 48 hr of separation from the mated queen and developed vitellogenic ovaries (on the left of each ovary, a pair of forceps holding them is shown)