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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Immunol Cell Biol. 2018 Mar 25;96(4):439–445. doi: 10.1111/imcb.12025

Figure 2.

Figure 2

Suppression of T cell proliferation by LysoPS requires GPR174 and Gαs. (a) Sorted naive CD4+ T cells were labeled with CFSE and incubated with plate-bound anti-CD3 and anti-CD28 for three days. On the left, histograms show the proliferation of wild-type (shaded, gray) and Gpr174−/Y (solid line, open) T cells cultured in the presence of the indicated concentration of 18:1 LysoPS. On the right, the division index of the cells is shown. (b) Experiments were carried out as in (A), except with Cd4creGnasfl/fl naive CD4+ T cells; * P < 0.05; ** P < 0.01. Data are representative of three independent experiments. Error bars indicate standard deviation for triplicate measurements.