Fig. 2.

Systemic antagomir-148a treatment efficiently inhibits miR-148a expression and results in upregulation of Bim protein in Th cells of the inflamed intestinal mucosa in colitic mice. Colitic Rag1-deficient mice were treated with antagomir-148a or antagomir-Scr as displayed in Fig. 1A. (A) On the last day of the experiment, CD3⁺CD4⁺ Th cells were isolated from the colonic laminae propriae by FACS. RNA from the sorted cells was purified and miR-148a expression was measured by TaqMan™ PCR. (B, C) Colonic Th cells were analyzed by flow cytometry following intracellular staining of the pro-apoptotic molecule Bim (B, C left panels) and the anti-apoptotic molecule Bcl-2 (B, C right panels). Shown are representative histograms (B) and the quantification of geometric means normalized to antagomir-Scr-treated mice (C). Data in (A) are pooled from two independent experiments with n = 10 and n = 8 mice, while data in (C) are pooled from two independent experiments with n = 12 and n = 13 mice that were treated with antagomir-148a or antagomir-Scr, respectively.