Supplemental Fig. 2.

The gut mucosae of colitic mice that were treated with antagomir-148a or antagomir-Scr show histopathological symptoms of colonic inflammation. Colitis was induced as shown in Figure 1A. For hematoxylin and eosin staining of the colons, the organs were fixed in 4% paraformaldehyde at 4 °C in the ‘‘Swiss roll’’ formation over-night. Next, the fixed colons were washed with PBS, dewatered and embedded in paraffin. In both treatment groups the inflamed colons show infiltration by immune cells, goblet cell loss, hyperplasia and crypt abscesses. Data are representative for two independent experiments.