Supplemental Fig. 4.

Antagomir-148a treatment during the effector phase of an acute immune response does not deplete antigen-specific memory Th cells and does not interfere with their effector function. Mice were immunized and injected with antagomirs as shown in Figure 4A or left untreated (non-immunized controls). Subsequently, mice were sacrificed to isolate splenocytes, which then were re-stimulated with NP-CGG and stained for flow cytometry. Shown are the absolute cell numbers of CD40L+ NP-CGG-specific Th memory cells (A) and the antigen-specific IFN-γ-expressing memory Th cells (B) following re-stimulation with NP-CGG. The data are from n=3 (non-immunized), n=4 (antagomir-Scr) and n=5 (antagomir-148a) mice, respectively.