Supplemental Fig. 6.

Injection with antagomir-148a during an acute immune response does not deplete FoxP3+ regulatory T cells. Mice were immunized and injected with antagomirs as shown in Figure 4A. Subsequently, splenocytes were isolated from the mice and stained for flow cytometric analysis. Depicted frequencies and absolute cell numbers of CD4+FoxP3+ regulatory T cells (Tregs) were measured by flow cytometry.