Supplemental Fig. 7.

Antagomir-148a treatment in the memory phase of an immune response does not deplete immunological T cell memory in primary and secondary lymphoid organs. Mice were immunized and injected with antagomirs as shown in Figure 5A. Afterwards, lymphocytes from the BM, the spleen and from the two inguinal lymph nodes (iLNs) were collected. (A-D) Cell numbers of memory CD4+ and CD8+ T cells expressing CD69 or Ly6C in the spleen (A, B) or in iLNs (C, D) were determined by flow cytometry. (E-J) Isolated cells from the BM (E, F), spleen (G, H) and iLNs (I, J) were stimulated with PMA/ionomycin prior to intracellular staining for IFN-γ and TNF-α. Cell numbers of cytokine-expressing cells were measured by flow cytometry.