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Culture, Differentiation and Immunophenotyping of ADSCs. (I) Representative image of ADSC displaying cell phenotype under bright-field microscopy (100×). (II-III) Stem cell pluripotency was evaluated by culture adipogenesis and osteogenesis under differentiation stimuli and posterior staining with (II) Oil-red-O and (III) Alizarin Red, respectively (100×). (IV-VI) Immunophenotypic characterization of ADSCs: (IV) CD29 (+), (V) CD45 (-) and (VI) CD90 (-).
