Fig 1.

EAE increased the activities of mitochondrial complex II and IV, but not complex I from the mouse renal cortex (A and B). Severe EAE had a trajectory to further augment the complex IV activity over mild EAE. This effect was associated with an increase of COX4 protein level (B and C). The activities of complex I and II were measured by the difference of absorption at A₆₃₀ between the assay buffer alone and assay buffer with 5 μg mitochondria extracts (A). The complex IV activity was assayed by increases in absorption at A₅₆₂ in the presence of 5 μg mitochondria extracts (B). The protein extracts from mouse kidney cortex were separated in a 4–12% Bis-Tris gel (Invitrogen) and probed with antibodies (C). (*p < 0.05 vs Control, p = 0.065 vs mild EAE; Control n = 7, Mild EAE n = 5, Severe EAE n = 6; One-way ANOVA for A and B, unpaired t test for C).