Fig 5. FTI-277 inhibits phosphate-induced apoptosis.

A) Human VSMCs were incubated in serum-free medium containing elevated phosphate (2.6 mM) ± FTI-277 (10 μM) or vehicle control. Cell lysates were collected 2 and 4 hours later and analysed by western blotting. (B) Human VSMCs incubated in serum-free medium containing phosphate (2.6 mM) ± FTI-277 (10 μM) ± SH6 (10 μM) for 12 hours were fixed and stained with DAPI. Apoptotic cells are expressed as a percentage of total cells (mean ± SEM); >750 cells were counted per variable. Data were analyzed by one-way ANOVA and Tukey post-hoc tests; *p<0.05.