Figure 2.
Native (non-denaturing) SEC/MS of conventional cysteine chemistry ADC: a) UV profile; b) zero-charge deconvoluted mass spectrum of intact ADC; c) zero-charge deconvoluted mass spectrum of de-N-glycosylated ADC. Abbreviations: a number LP denotes a number of LP moieties. *represent a method artifact that pertains to in-source fragmentation of LP.