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Reduced and alkylated peptide mapping of cysteine conjugated ADC using maleimide chemistry: a) zoomed view of total ion current for hinge conjugated peptide at pH 8.2 (top) and 7.5 (bottom); b) mass spectra of early eluting peak at pH 8.2 that corresponds tone drug conjugate peptide with thiosuccinimide ring opening (top) and one drug conjugated peptide without thiosuccinimide ring opening (bottom). Abbreviations: H denotes heavy chain peptide.
