(
A) No significant difference was found in the functional distribution of all HSN expressed genes compared tothe subset og genes with HSN regulatory signature or HSN expressed genes with conserved signature (chi-squared test computing p-values by Monte Carlo simulation p-value=0.59). (
B) Reporter analysis to test functionality of HSN signature windows in HSN expressed genes. Black lines represent the coordinates covered by bioinformatically predicted HSN signature windows (indicated by w and a number). Light blue lines indicate the published reporter construct(PMID:18408008, PMID: 15177025, PMID: 10926783 and PMID:19675228) . Green lines mark the region used in our analysis. Dark blue bar profiles represent sequence conservation in
C. briggsae, C. brenneri,
C. remanei and
C. japonica. See
Figure 7—source data 1 for a list of all reporters and raw scoring data.