(
A) pvclust was performed after removing 5HT pathway genes (
cat-1, tph-1, bas-1 and cat-4) from the HSN to show that proximity between HSN and mouse raphe is not only due to 5HT pathway genes. (
B) Clustering of worm neurons and mouse non-serotonergic control regions [cortical neurons from (
Molyneaux et al., 2015) show that HSN expression data cluster with raphe transcriptome is specific as it does not occur with other mouse transcriptomic data. P1_cpn: Postnatal day 1 cortical projection neurons; P1_corticothal: Postnatal day 1 cortical thalamic neurons; P1_subcereb: Postnatal day 1 cortical subcerebral neurons. (
C) 100 simulated 'HSN-like' neurons were obtained by assigning to each one the four 5HT pathway genes (
cat-1, tph-1, bas-1 and
cat-4) and 92 additional genes at random. None of these random profiles shows close proximity to the Raphe mouse neurons.