Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers |
|---|---|---|---|
| Strain, strain background (Caenorhabditis elegans) | C. elegans: Strain N2 | Caenorhabditis Genetics Center | WormBase: N2 |
| Strain, strain background (Caenorhabditis elegans) | Strain names and genotypes | Supplementary file 3 | |
| Strain, strain background (Mus musculus) | Mouse: C57Bl/6JRccHsd strain | ENVIGO, Harlan. (Huntingdon, Cambridgeshire, UK) | C57Bl/6JRccHsd |
| Strain, strain background (Escherichia coli) | Strain OP50 | Caenorhabditis Genetics Center | OP50 |
| Strain, strain background (Escherichia coli) | Rosetta 2(DE3) Singles Competent Cells: BL21 derivatives | Novagen, Merck Group. (Darmstadt, Germany) | Cat#71400 |
| Strain, strain background (Escherichia coli) | Strain: HT115(DE3) | Caenorhabditis Genetics Center | HT115 |
| Cell line (human) | Human: HEK293T | Laboratory of Oliver Hobert | ATCC: CRL-3216 |
| Transfected construct (C.elegans) | Plasmid: pCDNA3-egl-18 | This paper | N/A |
| Antibody | Mouse anti-GFP IgG1K | Sigma Aldrich, Merck Group. (Darmstadt, Germany) | Cat#11814460001 |
| Antibody | Anti-6X His tag antibody [HIS.H8] | Abcam (Cambridge, UK) | Cat#ab18184 |
| Antibody | Rabbit anti-5HT | Sigma Aldrich | S5545 |
| Antibody | Goat anti-5HT | Abcam | Ab66047 |
| Antibody | Rabbit anti-Sall2 | Sigma Aldrich | sc-6029 |
| Antibody | Alexa 555-conjugated donkey anti-rabbit | Molecular Probes, Invitrogen (Eugene, OR) | A-31572 |
| Antibody | Alexa 555-conjugated donkey anti-goat | Molecular Probes | A-21432 |
| Antibody | Alexa 488-conjugated donkey anti-rabbit | Molecular Probes | A-21206 |
| Antibody | Alexa 488-conjugated donkey anti-goat | Molecular Probes | A-11055 |
| Recombinant DNA reagent | Plasmid: pPD95.75 | Dr Oliver Hobert Laboratory | Addgene Plasmid #1494 |
| Recombinant DNA reagent | Plasmid: pRF4 (rol-6 (su1006)) | (Mello et al., 1991) | N/A |
| Recombinant DNA reagent | Plasmid: ttx-3prom::mcherry | (Bertrand and Hobert, 2009) | N/A |
| Recombinant DNA reagent | Plasmid: pBluescript | Dr Oliver Hobert Laboratory | N/A |
| Recombinant DNA reagent | Plasmid pJJR82 | Dr Mike Boxem Laboratory | Addgene #75027 |
| Recombinant DNA reagent | Plasmid pDD162 | Dr Mike Boxem Laboratory | Addgene #4754 |
| Recombinant DNA reagent | Plasmid pDD268 | (Dickinson et al., 2015) | N/A |
| Recombinant DNA reagent | Plasmid pJW1219 | (Ward, 2015) | Addgene # #61250 |
| Recombinant DNA reagent | Plasmid pCFJ90 | Dr Mike Boxem Laboratory | Addgene #19328 |
| Recombinant DNA reagent | Plasmid pPD129.36 (L4440) | Dr Andrew Fire Laboratory | Addgene #1654 |
| Recombinant DNA reagent | Plasmid: pET-21b-ast-1 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: pET-21b-unc-86 | (Zhang et al., 2014) | N/A |
| Recombinant DNA reagent | Plasmid: HSNearlyprom::ast-1 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: HSNearlyprom::hlh-3 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: HSNearlyprom::ast-1, HSNearlyprom::hlh-3 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: bas-1prom::ast-1 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: bas-1prom::Pet1 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: cat-4prom::hlh-3 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: cat-4prom::Ascl-1 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: cat-4prom::egl-46 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: cat-4prom::Insm1 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: cat-4prom::Gata2 | This paper | N/A |
| Recombinant DNA reagent | Plasmid: kal-1prom::Sall2 | This paper | N/A |
| Sequence-based reagent | oligonucleotides | Supplementary file 4 | |
| Commercial assay or kit | HisTrap HP Column | GE Healthcare Life Sciences (Marlborough, MA) | Cat#17-5248-01 |
| Commercial assay or kit | QuikChange XL Site-Directed Mutagenesis Kit | Agilent (Santa Clara, CA) | Cat# 200516 |
| Chemical compound, drug | EasyTides Adenosine 5'-triphosphate (ATP [γ−32P]) | Perkin Elmer (Waltham, MA) | Cat#NEG502A250UC |
| Chemical compound, drug | Power Broth Medium | Molecular Dimensions (Maumee, OA) | Cat#MD12-106-1 |
| Chemical compound, drug |
Lipofectamine 2000 | Invitrogen (Carlsbad, CA) | Cat#11668027 |
| Chemical compound, drug | Isopropyl-β-D-thiogalactopyranoside (IPTG) | Acros Organics, ThermoFisher Scientific (Waltman, MA) | Cat#BP1755-100 |
| Chemical compound, drug | Collagenase type IV | Sigma Aldrich | C-5138 |
| Chemical compound, drug | FluorSaveReagent | Merck Millipore (Darmstadt, Germany) | 345789–20 ML |
| Software, algorithm | Gorilla | (Eden et al., 2009) | http://cbl-gorilla.cs.technion.ac.il |
| Software, algorithm | R | (Team, 2016) | https://www.r-project.org/ |
| Software, algorithm | Bioconductor | (Huber et al., 2015) | https://www.bioconductor.org/ |
| Software, algorithm | pvclust (R package) | (Suzuki and Shimodaira, 2006) | www.sigmath.es.osaka-u.ac.jp/shimo-lab/prog/pvclust |