Fig. 3.

CXCL9/10/11 induced the activation of STAT3 and Akt. a: SGC7901 cells were incubated with CXCL9/10/11 (100 ng/mL) for the indicated times respectively, the phosphorylation of STAT3, Akt and ERK were analyzed by Western blot. b: SGC7901 cells were pretreated with or without AMG487 (1 μM) for 2 h followed by CXCL9/10/11 (100 ng/mL) stimulation for 5 min, cell lysates were collected for Western blot analysis. Normalized protein expression levels were calculated and analyzed. The gels were run under the same experimental conditions. The band intensities were calculated using the ImageJ 1.46r software. GAPDH was used as an internal control for the total protein measurement. The ratio of the target gene to GAPDH was used to conduct the statistical analysis. *P < 0.05 and **P < 0.01, as determined by Student’s t-test