Fig. 2A-B.

Annexin V/PI staining of fibroblasts and endothelial cells was performed after stimulation of normal saline, soap, or normal saline-EDTA. (A) Fibroblasts were stimulated with normal saline (97.2% viability), soap (33.4% viability), or normal saline-EDTA (97.6% viability) for 15 minutes. (B) Likewise, endothelial cells were stimulated with normal saline (97.3% viability), soap (27.6% viability), or normal saline-EDTA (97.7% viability) for 15 minutes. Annexin V/PI staining was conducted and detected by flow cytometry after 6 hours.