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. 2018 Apr 20;97(16):e0455. doi: 10.1097/MD.0000000000010455

Figure 1.

Figure 1

Gene transduction of αCD19 CAR and in vitro expansion of primary T cells. A, In vitro expansion of T cells following activation with αCD3/αCD28 coated magnetic beads on day 1 and transduction of CAR-encoding lentiviral vector on day 0. B, Representative FACS plots showing the proportion of CCR7+/CD45RA+ T cells (stem memory T cells). C, αCD19-specific CAR surface expression in T cells. The data shown in (B) and (C) were examined 5 days following transduction with CAR-encoding lentiviral vector. CAR = chimeric antigen receptor, FACS = fluorescence activated cell sorting.