Fig. 1.

Analysis of the methylation status of the PTENP1 pseudogene 5’-terminal region by methyl-sensitive PCR. U and m are products of PCR amplification with primers to an unmethylated or methylated template, respectively. Bisulfite-treated DNA templates: uC – a peripheral blood sample (unmethylated control); mC – a peripheral blood sample methylated by SssI methylase (methylated control); 1–3 – tumor DNA samples with (1) methylated PTENP1, (2) unmethylated PTENP1, and (3) partially methylated PTENP1 (some of the tumor cells had unmethylated PTENP1); C0 – amplification without a DNA template; M – a molecular weight marker