Fig. 1.

Maps of the expression plasmids p1.1-F9, p1.2-Zeo-VKORC, and p1.2-Hyg-Fur. pCHO EEF1A – functional promoter of the gene of Chinese hamster translation elongation factor 1 alpha, the 5’ untranslated region of this gene and nontranscribing DNA regions flanking this gene; IRES – internal ribosome binding site of the EMCV; DHFR – the open reading frame of the mouse DHFR gene used for selection and genome amplification in eukaryotic cells; tCHO EEF1A – polyadenylation signal, the transcription terminator of the Chinese hamster translation elongation factor 1 alpha gene and the corresponding 3’ nontranscribing DNA region flanking the aforesaid gene; FIX – open reading frame of the human blood clotting factor IX; VKORC – open reading frame of the Chinese hamster VKORC1 gene; SV40 prom – immediate early promoter of the SV40 virus; SV40t pA – transcription terminator and polyadenylation sequence of the SV40 virus; Zeo – open reading frame of the Sh ble gene (Streptoalloteichus hindustanus bleomycin) conferring zeocin resistance; Furin – open reading frame of the human PACE/furin protease gene; Hygro – open reading frame of the hygromycin phosphotransferase gene (E.coli hpt). Promoter directions are depicted by arrows