Fig. 5.
SDS-PAGE analysis of the chromatography fractions obtained during the purification of FIX. Electrophoresis was performed under nonreducing conditions; molecular weights are given in kDa; colloidal Coomassie staining. Denotation: M – marker, eluate – the elution fraction from the corresponding column; flow-through – the flowthrough fractions. The corresponding NaCl concentration in the elution solutions are given for the elution fractions from the Capto Q column; CaCl2 concentration was 10 mM for all lanes