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The VKORC1 activity level in stably transfected cells
| Plasmid |
Specific VKORC1 activity, % of substrate conversion per 1 mg/ml of total protein in the lysate for 1 h |
The relative level of increase in VKORC1 activity, times |
|---|---|---|
| Intact CHO DG44 cells | 0.38 | - |
| p1.2-Zeo-VKORC1 | 9.21 | 24.2 |
| p1.2-Zeo-hVKORC1 | 3.03 | 8.0 |
Note: specific activities were calculated for the linear regions of the curve showing substrate conversion versus total protein concentration in lysates.
