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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Microbiol. 2017 Nov 17;106(6):1018–1031. doi: 10.1111/mmi.13865

Figure 3. pyc::tn is defective in late-stage biotin biosynthesis.

Figure 3

(A) pathway of late-stage biotin biosynthesis. Pimeloyl-CoA is converted to biotin via the action of four biotin biosynthetic enzymes: BioF, BioA, BioD, and BioB. Expression of bioFD and of bioB, but not of bioA, has been shown (Tang et al., 2014) to be repressed by the TetR family repressor BioQ under biotin-replete conditions in M. smegmatis. (B, C) WT (MGM8019), bioA::tn (MGM8021), and pyc::tn (MGM8020) were tested for growth on medium containing (B), or lacking biotin (C, left panels) or containing the biosynthetic intermediate desthiobiotin (DTB) (C; two rightmost panels). bioA::tn and pyc::tn strains expressing bioA (pyc::tn: (MGM8034)); bioA::tn: (MGM8036) or bioB (pyc::tn: (MGM8035); bioA::tn: (MGM8037)) under the control of a heterologous promoter responsive to the inducer anhydrotetracycline (ATc) (C; ATc present in bottom panels) were also tested.