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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Microbiol. 2017 Nov 17;106(6):1018–1031. doi: 10.1111/mmi.13865

Figure 4. Pyruvate carboxylase is required for BioQ mediated derepression of bioFDB.

Figure 4

(A) Expression of the biotin biosynthetic genes bioFADB in WT (MGM8007), pyc::tn (MGM 8008) and complemented (MGM8011) strains under biotin-replete and biotin-deficient (-bio) conditions. Strains were grown for three hours with or without biotin, RNA was prepared, and RT-qPCR was performed for the indicated mRNAs. (B) Loss of bioQ suppresses the biotin auxotrophy of pyc::tn. Genetic deletions of pyc (MGM6514) and bioQ (MGM6516 and MGM6520) were introduced via homologous recombination and double counterselection (see strain table), and growth on medium containing or lacking biotin was assessed. (C) Loss of bioQ restores bioB expression to pyc::tn. Error bars are standard deviation of three biological replicates.