Table II.
Specificity of APase promoter induction and chemical inductiona
| Days after Treatment | Relative GUS Expressionb
|
||||
|---|---|---|---|---|---|
| SA | JA | MN | MN | MN | |
| 100 μm | 5 mm | 20 mm | 50 mm | ||
| 1 | − | − | − | + | ++ |
| 3 | + | − | − | + | +++ |
| 5 | − | − | + | + | ic |
| 7 | +− | − | − | ++ | i |
The experiment was repeated twice with similar results. For each treatment and sampling time 10 T2 seedlings that were the progeny of the GUS6 transformant T1 line were evaluated. Little variation in GUS expression was observed within each treatment and sampling time.
Arabidopsis seeds were germinated in washed sand and fertilized with 0.25× Hoagland solution. SA, JA, or mannose (MN) at the indicated concentrations were gently sprayed onto the 14-d-old seedlings until run off and were added to the sand (concentrated solution to reach the same concentration in the sand solution as in the overhead spray).
GUS expression evaluated as in Table I.
Indicates that seedlings were too injured by treatment to be evaluated.