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. 2018 Apr 24;18:63. doi: 10.1186/s12935-018-0557-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Cell viability assessment upon single and combined treatments. Trypan-blue exclusion assay was applied for the evaluation of the cell viability 24 h post-treatment with single RTKi or in combination with CQ and SP1. The percent change measured with respect to DMSO control (DMSO percent change = 0) is shown. Results are presented as mean ± SD out of triplicates, and p value marked as **p < 0.01; ns non-significant (Dunnett’s test done using CQ value as a control for the multiple comparison). CQ Chloroquine, SP1 Spautin-1, Af Afatinib, Sor Sorafenib