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. 2018 Apr 24;18:63. doi: 10.1186/s12935-018-0557-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Autophagic vacuoles creation. Autophagy activation upon treatment with Afatinib was additionally validated using immunofluorescence in order to visualize a creation of autophagy vacuoles; ×60 magnification. Green—autophagy vacuoles; Blue—Hoechst, nuclear staining. The combined treatment with Afatinib and a CQ or b SP1 was used