Figure 5.

CARMEN Transcripts Are Differentially Regulated During Fetal and Adult Differentiation Into Smooth-Muscle Cells Or Cardiomyocytes

(A) UCSC screenshot of the human CARMEN locus. The 10 CARMEN isoforms, that is, CARMEN1, 2, and 3, 4, 5, 6, 7, 8, 9, and 10 are indicated in red. The 2 miRNAs, MIR-143/145, are indicated in green. (B) Expression of CARMEN transcripts (CARMEN1, 2, 3, 6, 7, 8, 9, 10) in proliferating (Expansion; white), and in differentiated fetal and adult CPCs without prior DLL1 stimulation and cultured in the absence of DAPT (Differentiation; black) or after stimulation with DLL1 and cultured in presence of DAPT (DLL1/DAPT/Differentiation; light gray). Data represent mean ± SEM; *p < 0.05 as compared with proliferating CPCs (n = 4). (C) Expression of CARMEN7, MIR-143, MIR-145, ELK1, and KLF4 transcripts in proliferating CPCs (Expansion [Exp]; black) and in differentiated adult CPCs (white and light gray), exposed or not to DLL1 in the absence or presence of DAPT. Data represent mean ± SEM; *p < 0.05 as compared with proliferating CPCs; ‡p < 0.05 as compared with differentiating unstimulated (None; white) CPCs in absence of DAPT (n = 3).