Table 1.

Transcriptome Sequencing Details. RNA yields ranged from 3.7 to 64.0 µg of purified RNA for beetles across developmental life stages. Sequencing was performed on an Illumina® NextSeq 500. Overall paired-end reads mapping rates of individual sample libraries are included. The representative transcriptome has been filtered for ribosomal RNA, noncoding RNA, microbial and plant contaminating RNA, and mitochondrial sequences; therefore, any reads in the individual libraries mapping to these filtered sequences would not map to the representative transcriptome. bp: base pair, GC: G-C nucleotide ratio

Tissue	Reads	Bases (bp)	GC Content	Mapping Rate
Eggs	39,722,314	5,798,921,254	46%	90.5%
First instar larvae	127,028,854	18,509,700,969	45%	89.3%
Second instar larvae	35,479,130	5,167,790,213	46%	85.2%
Third instar larva	39,298,572	5,720,171,102	46%	87.0%
Pupa	111,438,838	16,278,597,449	45%	85.0%
Non-breeding female adult	103,768,508	15,199,597,722	42%	79.4%
Total	456,736,216	66,674,778,709	45%	86.1%