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. 2018 Apr 25;6:13. doi: 10.1038/s41413-018-0014-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Anti-LTA mAb characterization. Experimental anti-LTA antibody (SAC55) was analyzed for binding characteristics by several methods against species-matched isotype control IgG (R347). a k_off, k_on, and K_D were assessed by Octet for SAC55 against LTA. b Selective binding of SAC55 to Gram-positive bacteria (S. aureus and S. epidermidis) but not Gram-negative bacteria (E. coli and P. aeruginosa) is assessed, as well as an absence of binding by the control antibody R347. c Flow cytometry of in vitro grown S. aureus reveals selective binding, with the distinction between groups improved by addition of human sera. d Flow cytometry of S. aureus harvested from mouse blood shows selective binding of SAC55 to Gram-positive bacteria, following in vivo passage.