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. 2018 Apr 25;8:6523. doi: 10.1038/s41598-018-24654-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of sodium cromolyn injection on acupuncture analgesia. Pain threshold was normalized according to pain thresholds determined prior to establishing the AA model; the data are presented as the mean ± s.e.m. in the figure. On day 1, the AA model was established. Before establishing the model, the pre-modelling pain threshold was measured. On day 3, first, the post-modelling pain threshold was measured, and the post-treatment pain threshold was measured 20 min after treatment. For the ACU group, acupuncture was performed at the ST36 acupoint for 20 min. For the CRO + ACU group, sodium cromolyn solution was injected locally at the acupoint 5 min before acupuncture. The Model group was restrained for 20 min. Sodium cromolyn was found to inhibit the analgesic effect induced by acupuncture in AA model rats. * vs ACU group, P < 0.05.