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. 2018 Apr 25;8:6523. doi: 10.1038/s41598-018-24654-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Acupuncture-induced change in adenosine at rat ST36. A microdialysis probe was used to collect tissue fluid specimens at the acupoint, and adenosine concentrations were measured using HPLC. Each data point represents the mean ± s.e.m. of the adenosine concentration in the specimen collected at 30-min intervals. The ACU group was administered 30 min of acupuncture, as represented by the shadow. For the CRO + ACU group, sodium cromolyn solution was injected at the acupoint 5 min before acupuncture, which is represented by the dotted line. Sodium cromolyn was found to inhibit an increase in the adenosine concentration triggered by acupuncture. *vs ACU group, P < 0.05.