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. 2018 Apr 25;8:6519. doi: 10.1038/s41598-018-24628-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Forced expression of miR-9 inhibits G1ER cell differentiation. (A) qRT-PCR analysis of miR-9 precursor expression in Migr1 and Migr1-MiR-9 transduced G1ER cells. Hprt gene was used for normalization. (B) Representative images of the benzidine staining of GFP⁺ G1ER cells expressing EGFP or miR-9/EGFP. Differentiation of the cells was induced with β-estrodial for two days. (C) The diagram shows the average percentage of Benzidine positive cells in GFP⁺ G1ER cells infected with control vector and miR-9 in three independent experiments. *P < 0.05.