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. 2018 Apr 25;8:6519. doi: 10.1038/s41598-018-24628-0

Figure 3.

Figure 3

Forced expression of miR-9 inhibits differentiation and reduces the frequency of cell death of early erythroid progenitor cells. Day 14.5 fetal liver cells were infected with Migr1 vector or Migr1-miR-9, and EGFP+ cells sorted and plated on methylcellulose medium. (A) Histograms showing the number of BFU-E colonies. (B) Histograms showing the number of CFU-E colonies. (C) The representative BFU-E colonies formed by Vector and MiR-9 infected fetal liver cells. (D) Representative image of benzidine staining of cells from BFU-E colonies. *P < 0.05; ***P < 0.001. (E) Representative histograms of flow cytometric analysis of apoptotic and dead cells in vector or miR-9 infected(GFP+) fetal liver cells two days after induction of differentiation in vitro. (F) The percentage of DAPI+ cells in vector or miR-9 infected fetal liver cells. Experiments were repeated three times. **P < 0.01.