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. 2018 Apr 25;8:6519. doi: 10.1038/s41598-018-24628-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-9 inhibits erythroblast cell differentiation in vivo. (A) The histogram shows the percentage of Ter119+ cells in gated EGFP+ cells in vector and miR-9 chimeric mice. To generate the chimeric mice, the Lin^- primary bone marrow cells were infected with retrovirus expressing miR-9 or control vector, followed by transplanted into lethally-irradiated wildtype recipient mice. (B) Flow cytometric analysis of the frequencies of erythroblasts in bone marrow cells from the representative vector and chimeric mice. (C) Histogram shows the average frequencies of erythroblasts in vector and miR-9 chimeric mice (means ± SD, n = 5–7). *P < 0.05; ***P < 0.001.