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. 2018 Apr 25;8:6519. doi: 10.1038/s41598-018-24628-0

Figure 5.

Figure 5

Ectopic expression of miR-9 increases production of ROS in fetal liver cells and G1ER cells. (A) Representative histograms show ROS levels in GFP + fetal cells infected with Migr1 or Migr1-miR-9. Top panel, untreated cells; bottom panel, cells treated with NAC. (B) Summary of ROS levels in Migr1 and Migr1-miR-9 transduced fetal liver cells without treatment (left panel) or with NAC treatment (right panel). (C) qRT-PCR analysis of the expression of ROS scavenging enzymes in vector or miR-9 infected fetal liver cells. Experiments were repeated three times. (D) Representative histograms show ROS levels in GFP+G1ER cells infected with Migr1 or Migr1-miR-9. Top panel, untreated cells; bottom panel, cells treated with NAC. (E) Summary of ROS levels in Migr1 and Migr1-miR-9 transduced G1-ER cells without treatment (left panel) or with NAC treatment (right panel). (F) qRT-PCR analysis of the expression of ROS scavenging enzymes in vector or miR-9 infected G1ER cells. *P < 0.05.